Biotechnology Principles and Processes - Online Test
Q1. During agarose gel electrophoresis DNA fragments moves towards anode. This is because
Answer : Option B
Explaination / Solution:
To separate DNA using agarose gel electrophoresis, the DNA is loaded into pre-cast wells in the gel and a current applied. The phosphate backbone of the DNA (and RNA) molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode.
Q2. Rop genes in pBR322 codes for
Answer : Option A
Explaination / Solution:
The plasmid pBR322 contains a Rop gene coding for the Rop protein, which promotes conversion of the unstable RNA I – RNA II complex to a stable complex.
Rop genes in pBR322 codes for protein involved in replication of plasmid. Plasmid are able to take the foreign gene and to be transferred to target cells.
Q3. The particles used to coat with DNA in Biolistic gun is of
Answer : Option B
Explaination / Solution:
DNA is coated with tungsten before used in biolistic gun for inserting the DNA directly into target cells for obtaining particular protein.
Q4. Production of large scale recombinant products can be done in
Answer : Option B
Explaination / Solution:
Bioreactor is a large vessels in which large scale raw materials are biologically converted into specific products.
Bioreactor is used to produce large scale production.
Q5. DNA fragments move at different distances in Gel Electrophoresis because
Answer : Option C
Explaination / Solution:
Gel electrophoresis is a technique used to separate DNA fragments (or other macromolecules, such as RNA and proteins) based on their size and charge. Electrophoresis involves running a current through a gel containing the molecules of interest. Based on their size and charge, the molecules will travel through the gel in different directions or at different speeds, allowing them to be separated from one another.
During gel electrophoresis different fragments move at different distances due to difference in their molecular weight of the fragments. Smaller fragments move more towards anode.
Q6. The palindromic sequence out of the given options is
Answer : Option D
Explaination / Solution:
Palindromic sequence is the sequence which reads same from left to write or from right to left. MADAM is an example of palindromic sequence.
Q7. EtbR fluoresces in UV light because
Answer : Option A
Explaination / Solution:
Ethidium bromide is a molecule commonly used to visualize DNA in agarose gel electrophoresis experiments. It both binds to DNA and fluoresces under the proper conditions. Ethidium bromide is known as an intercalating agent. The flat structure of ethidium bromide allows it to intercalate, or insert, between nitrogenous bases of a DNA molecule. This interaction is especially useful because of ethidium bromide's second characteristic. When it is exposed to ultraviolet light, ethidium bromide fluoresces. Thus, this chemical provides both a means of tagging DNA molecules and a means of visualizing them.
Q8. Elution can be done by using the enzyme
Answer : Option A
Explaination / Solution:
Elution is the process of extracting one material from another by washing with a solvent. Elution can be done by using enzyme agarase that can catalyze the hydrolysis of agar.
Q9. Technique associated with DNA amplification is
Answer : Option C
Explaination / Solution:
DNA amplification is done by using the technique of polymerase chain reaction in which millions of copies of DNA segments are produced.
Q10. The DNA fragments in an agarose gel at the same position signify that the two fragments have
Answer : Option C
Explaination / Solution:
Gel electrophoresis is a technique used to separate DNA fragments (or other macromolecules, such as RNA and proteins) based on their size and charge. Electrophoresis involves running a current through a gel containing the molecules of interest. Based on their size and charge, the molecules will travel through the gel in different directions or at different speeds, allowing them to be separated from one another.
The DNA fragments in an agarose gel at the same position signify that the two fragments have same molecular weight and travel same distance.
